.Multiplexed PCR in one tube with numerous different type of primers that bound on tube bottom surface at specific site to each other primers one strand of sense or antisense and detected by hybridization with labelled Probe.

摘要

The present invention, each mounting a respective given position difference variety of Primer to the bottom surface of a diameter of 4mm Tube 0.2ml PCR for the production and the floor is flat ln reaction solution prepared for PCR reaction attached to the bottom Tube Primer and correspondingly it gives put (mibuchak Primer on the following solution) Primer that can perform the PCR reaction. It gives to occur in a nucleic acid sample and a PCR reaction to be analyzed with these. After the PCR reaction solution was treated with alkali to remove the PCR product synthesized by mibuchak Primer on the solution, to give Hybridization and PCR product formed by the Tube Primer attached to the floor by applying a marker is attached Probe herein. The Probe is a nucleic acid does not fulfill the binding reaction and consists of a base sequence within the nucleic acid product of the non-specific PCR reaction in PCR. Experimental techniques for analyzing the genetic information of the test results in a way that leverages the reading mechanism such as a Laser scanner confirms the position of the markers in the floor of the attached PCR Tube Probe.