Synthetic secretory signal sequences and methods for producing foreign proteins using the same

摘要

The present invention relates to an artificial secretory signal sequence and a method for producing an exogenous protein using the same. More specifically, the present invention relates to a novel synthetic secretion signal sequence consisting of 23 amino acids, a recombinant plasmid containing the same, and a gene encoding an exogenous protein inserted into the plasmid and introducing the gene into Escherichia coli to culture the transformant And inducing expression of the recombinant Escherichia coli, thereby efficiently producing an exogenous protein outside the cytoplasm of the recombinant Escherichia coli. The recombinant plasmid of the present invention shows a very high protein secretion efficiency in various E. coli and various expression systems. If a specific exogenous protein is mass produced using this point, secretion into the surrounding cytoplasm is possible, The cost and time can be considerably reduced, and an economical production of recombinant proteins can be achieved from an industrial point of view. In addition, since the recombinant plasmid of the present invention can directly link the synthetic secretory signal sequence and the gene of the foreign protein to be secreted without a linker, unnecessary amino acid is not inserted into the secreted foreign protein, which is very advantageous for the production of medical proteins.