RECOMBINANT MICROORGANISMS EXPRESSING ANTIGENIC PROTEINS OF HELICOBACTER PYLORI

摘要

PURPOSE: Provided are chimeric proteins consisting of antigenic proteins of Helicobacter pylori and A2 and B subunits of Vibrio cholerae toxin, more specifically, recombinant DNAs coding for antigenic proteins of Helicobacter pylori and A2 and B subunits of Vibrio cholerae toxin, recombinant expression vectors containing the genes, a process for preparing the chimeric proteins employing the recombinant microorganisms transformed with the expression vectors, and preventive and therapeutic vaccines comprising the chimeric proteins for Helicobacter pylori-associated diseases. CONSTITUTION: The recombinant DNA comprises a fusion gene which is prepared by ligating antigenic determinant coding gene of Helicobacter pylori and A2 and B subunit genes of Vibrio cholerae toxin, wherein the antigenic determinant coding gene of Helicobacter pylori is selected from the group consisting of ureB, cagA, alpA, alpB, fliQ, babA1, babA2, ureC, ureD, ureA, sodB, ureI, ureE, ureF, ureG, ureH, flaA, flaB, catA, vacA and babB.