New Corynebacterium glutamicum GTP cyclohydrolase I, dihydropteroate synthase, dihydroneopterin aldolase and 2-amino-4-hydroxy-6-hydroxymethyl-dihydropteridine pyrophosphokinase polypeptides

摘要

Corynebacterium glutamicum GTP cyclohydrolase I, dihydropteroate synthase, dihydroneopterin aldolase and 2-amino-4-hydroxy-6-hydroxymethyl-dihydropteridine pyrophosphokinase polypeptides, are new. The following polypeptides are new: (i) a polypeptide with GTP cyclohydrolase I activity having a defined sequence of 202 amino acids given in the specification, optionally with one or more amino acid deletions, insertions or substitutions; (ii) a polypeptide dihydropteroate synthase activity having a defined sequence of 285 amino acids given in the specification, optionally with one or more amino acid deletions, insertions or substitutions; (iii) a polypeptide with dihydroneopterin aldolase activity having a defined sequence of 131 amino acids given in the specification, optionally with one or more amino acid deletions, insertions or substitutions; and (iv) a polypeptide with 2-amino-4-hydroxy-6-hydroxymethyl-dihydropteridine pyrophosphokinase activity having a defined sequence of 160 amino acids given in the specification, optionally with one or more amino acid deletions, insertions or substitutions. Independent claims are also included for the following: (1) a polynucleotide encoding one of the polypeptides; (2) a gene construct comprising at least one copy of the polynucleotide of (1) together with at least one regulatory sequence; (3) a host organism transformed with the gene construct of (2); (4) production of folic acid by culturing the organism of (3).