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For the rapid sequencing of genomes the DNA is linearized automatically and at reduced costs with a mixture of proteases
For the rapid sequencing of genomes the DNA is linearized automatically and at reduced costs with a mixture of proteases
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机译:为了对基因组进行快速测序,可将DNA自动与蛋白酶混合物线性化并降低成本
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摘要
Rapid sequencing of genomes by linearizing DNA, isolated genome DNA or un-isolated DNA sequence is placed on a longitudinal plate in a mixture of anaphase-, telophase-, G phase- or mitosis- or interphase chromosomes (10), broken down by proteases. It can also be laid on a rule or in a straight line along a rod or linearized. The DNA sequence is sequenced by laser scanning microscopy, weak ultra violet radiation absorbed by the DNA, screen tunnel microscopy, electron microscopy (EM), nuclear magnetic resonance (NMR) or other spectrographic analysis, circular dichroic (CD), and the like. The substrates can be moved on the long plate in opposite directions. Both ends of the genome sequence (7) are spliced with the free ends of the sequences immobilized in the recesses on the substrate, by the addition of DNA ligates and androstane triphosphate (ATP). As the movement of the substrate is in opposite directions, the sequences on the long plate are linearized on its surface or along the grooves. The sequences are immobilized on the substrate. The interphase are spliced by acting on the telome to release telome DNA sequences. The chromosome proteins are reduced or degraded in a mixture of proteases (11), without damage to the DNA. The surface of the long plate can be coated with a mixture of helicase such as 3'-5' E. Coli Rep helicase and ATP so that a double-strand DNA is divided into two single strands. The long plate can be elastic or rigid. During de condensation and linearizing the chromosome DNA is irradiated with bio photons, the photons are then detected by photo-sensors such as photo-multipliers. The detection signals are stored and/or processed. The long plate has the groove and/or recess or reagent glass. The long plate or only the recess can be heated to release hydrogen bridges or DNA-DNA interactions. They can also be released by the addition of a denaturing material. A number of fragments of genome or chromosome DNA sequences (7) can be sequenced on the long plate simultaneously, in a multi-parallel sequencing action, in an automatic process. The movement of the substrate along the long plate is controlled automatically.
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