Refolding membrane proteins into their native or active form comprises solubilizing the protein in a first detergent and replacing the first detergent with a second detergent

摘要

Method for refolding membrane proteins into their native or active form comprises solubilizing the protein in a detergent then replacing the detergent with a second detergent selected from 8-16C alkyl N,N-methylglycines, 5-12C alkyl glycosides and thioglycosides, saccharide fatty acid esters, bile acids and their derivatives, glucamides, lecithins, lysolecithins and 10-16C alkyl phosphocholines. An Independent claim is also included for proteoliposomes comprising proteins produced by the method.