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New nucleic acid encoding plant receptor-like protein kinase, useful for identifying new herbicides and growth regulators

机译:编码植物受体样蛋白激酶的新核酸,可用于鉴定新的除草剂和生长调节剂

摘要

Nucleic acid (I) that encodes a plant polypeptide with the biological activity of a receptor-like protein kinase of sequence (2; 794 amino acids (aa)), reproduced. Independent claims are also included for the following: (a) regulatory regions (RR) that naturally control transcription of (I) in plant, especially tobacco, cells; (b) DNA construct containing (I) and a heterologous promoter; (c) vector containing (I), RR or the construct of (b); (d) host cells containing (I), the construct of (b) or the vector of (c); (e) polypeptide (II) with the activity of a receptor-like kinase encoded by (I) or having at least 60% identity with (2); (f) antibody (Ab) that binds specifically to (II); (g) method for preparing (I); (h) recombinant production of (II); (i) identifying compounds (A) that bind to (II); (j) identifying compounds (B) that alter expression of (II); (k) transgenic plants (or their derivatives) that, following insertion of (I), the construct of (b) or the vector of (c), have altered intracellular concentration of (II), relative to the wild-type; (l) plants (or derivatives) in which the biological activity or expression pattern of (II) differs from that in the wild-type; and (m) producing plants of (m) by endogenous mutation of (I) or RR.
机译:复制了编码植物多肽的核酸(I),该多肽具有序列(2; 794个氨基酸(aa))的受体样蛋白激酶的生物活性。还包括以下方面的独立权利要求:(a)自然控制植物,尤其是烟草细胞中(I)转录的调控区(RR); (b)含有(I)和异源启动子的DNA构建体; (c)含有(I),RR或(b)的构建体的载体; (d)含有(I),(b)的构建体或(c)的载体的宿主细胞; (e)具有由(I)编码的受体样激酶的活性或与(2)具有至少60%同一性的多肽(II); (f)与(II)特异性结合的抗体(Ab); (g)制备(I)的方法; (h)(II)的重组生产; (i)鉴定与(II)结合的化合物(A); (j)鉴定改变(II)表达的化合物(B); (k)在插入(I),(b)的构建体或(c)的载体后,相对于野生型改变了(II)的细胞内浓度的转基因植物(或其衍生物); (l)(II)的生物活性或表达方式与野生型不同的植物(或衍生物); (m)通过(I)或RR的内源突变产生(m)的植物。

著录项

  • 公开/公告号DE19961519A1

    专利类型

  • 公开/公告日2001-06-21

    原文格式PDF

  • 申请/专利权人 BAYER AG;

    申请/专利号DE1999161519

  • 发明设计人 SCHMUELLING THOMAS;SCHAEFER SILKE;

    申请日1999-12-20

  • 分类号C12N9/12;C07K16/00;A01H5/00;C12N15/63;C12N15/54;C12N5/10;C07H21/04;

  • 国家 DE

  • 入库时间 2022-08-22 01:10:05

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