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Genotyping multidrug resistance gene-1, useful for assessing doses of pharmaceuticals, by mass spectrometric analysis of primer extension products
Genotyping multidrug resistance gene-1, useful for assessing doses of pharmaceuticals, by mass spectrometric analysis of primer extension products
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机译:通过对引物延伸产物进行质谱分析,对多药耐药基因-1进行基因分型,可用于评估药物剂量
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摘要
Method for genotyping the MDR-1 (multidrug resistance-1) gene by mass spectrometric detection of the mutational status at some or all of 16 point mutations (single nucleotide polymorphism; SNPs). The sequences around all these mutations (coding and non-coding strands) are reproduced as sequences (1)-(32). Independent claims are also included for the following: (a) chemical package used to process samples for the new method comprising at least the primer for selective amplification of a DNA segment surrounding an SNP, reagents for primer extension and a mixture of terminating and non-terminating nucleotide triphosphates (NTPs); (b) point mutations defined by sequences (1)-(32); and (c) oligonucleotides, sequences (33)-(64), given in the specification, as attachment sites for primers in a mutation-dependent primer extension process for analysis of SNP.
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