Vaccines against conformation-dependent or non-peptide antigens, based on DNA encoding peptide which mimics the antigen, useful e.g. as antitumor vaccines

摘要

A vaccine (V1) against conformation-dependent antigens (CDA) comprising DNA (I) and/or an antibody, or peptide which immunologically imitates CDA, is new. A vaccine (V1) against conformation-dependent antigens (CDA) comprising DNA (I) and/or an antibody, or peptide which immunologically imitates CDA, is new. (I) encodes a region of an antiidiotypic antibody (Ab2) or another peptide which: (a) specifically binds to the binding site of an antibody (Ab1) or an antigen binding molecule; and (b) immunologically mimics the initial antigen. The epitope is partially or completely conformation-dependent, and has an immunogenic structure defined by a specific spatial conformation of amino acids. (I) is used in the form of linear or circular naked DNA and/or with a viral vector and/or adjuvants. Independent claims are also included for the following: (1) a corresponding vaccine (V2) against antigens which are not proteins or peptides, as defined above but which have epitopes which show an immunogenic structure; (2) preparing (V1) and (V2); (3) human antiidiotypic antibody fragments against the MUC1-conformation epitope having one of 31 approximately 60 residue amino acids sequences, all fully defined in the specification; (4) MUC1-conformation epitope mimics having one of 16 9-17 residue amino acid sequences, all fully in the specification; (5) antiidiotypic antibody fragments against the TF antigen having one of 24 approximately 200 residue amino acid sequences, fully defined in the specification; (6) TF carbohydrate epitope mimetics having one of 25 7-13 residue amino acid sequences, all fully defined in the specification; and (7) DNA sequences encoding the fragments and derivatives defined in (3, 4, 5, or 6).