Purified interleukin-1 and DNA encoding interleukin-1 and their preparation, such DNA-containing vectors and their preparation and use for transforming hosts which allow the expression of interleukin-1

摘要

(1)Interleukin 1 (I), as a homogenous protein of molecular wt. about 17500 daltons and having a partial amino acid sequence of formula (II), is new. (2)Homogeneous human interleukin 1 consisting of a single protein species of molecular wt. of about 17500 daltons and having the following amino acid compsn. is new. The compsn. (amino acid and no. of residues/molecule, consists of Asp/Asn 14; Thr 8; Ser 11; Glu/Gln 22; Pro 6; Gly 11; Ala 8; Cys at least 1; Val 11; Met 4; Ile 6; Leu 11; Tyr 4; Phe 11; His 3; Lys 13; Arg 3. (3) Pure DNA encoding for the expression of human (I) molecules is new. (4) Recombinant DNA cloning vector comprising a CDNA sequence comprising the gene for (I) is new. (5)Recombinant DNA cloning vector comprising DNA as claimed in paragraph (3) above is new. (6)Host transformed by a cloning vector as defined in paragraphs (4) and (5) above is new.