Producing high-genetic value embryos of specified sex, comprises thawing frozen solution of genetically-selected spermatozoa, sorting them into subpopulations, and fertilizing oocytes with subpopulation in vitro

摘要

Producing high-genetic value embryos of predetermined sex derived from gametes of non-human mammalian species, comprising thawing and diluting a frozen solution of genetically-selected spermatozoa (GS), staining GS with a DNA specific fluorescent dye, sorting GS into subpopulations (SP), in vitro homologous fertilization of oocytes with SP, and incubating fertilized oocytes in mammalian cell culture medium, is new. Producing high-genetic value embryos of predetermined sex derived from gametes of non-human mammalian species, comprising: (a) thawing a frozen solution of GS and diluting it; (b) staining the spermatozoa with a DNA specific fluorescent dye; (c) diluting out the stained sample; (d) sorting the stained spermatozoa population by flow-cytometry into a spermatozoa subpopulation (SP1) containing chromosome X and a spermatozoa subpopulation (SP2) containing chromosome Y, using a protein free solution as sheath fluid; in vitro homologous fertilization of oocytes with SP1 or SP2; and (e) incubation of fertilized oocytes into a mammalian cell culture medium, is new. An Independent claim is also included for a non-human high-genetic value mammalian embryo of predetermined sex, obtainable by the above said method.