Detecting interactions between oligonucleotides and RNA using fluorescence resonance energy transfer (FRET)

摘要

There is provided a device and method for detecting interactions between oligonucleotides immobilised on a solid array and RNA that is added to the array. This detection of the interaction relies on Fluorescence Resonance Energy Transfer (FRET) between the two chromaphores. As the precise sequence of the immobilised oligonucleotides is known, it is therefore possible to determine structural parameters of native RNA transcripts and infer regions that may be effective targets for antisense mediated gene knock-down.