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Fast cross-correlation frequency domain fluorescence measurement - phosphorescence measurement

机译:快速互相关频域荧光测量-磷光测量

摘要

PURPOSE: To obtain a cross correlation frequency range fluorescence measuring apparatus and/or phosphor measuring apparatus in which signals can be acquired at high sensitivity and high S/N ratio. ;CONSTITUTION: The inventive apparatus comprises an electromagnetic radiation source 10, means 12 for amplitude modifying an electromagnetic radiation at a first frequency, means for introducing a modulated electromagnetic radiation to a sample 20, and means 32 for detecting luminescence of the sample. The inventive apparatus further comprises means 26, 28 for supplying the detection means with a signal coherent to the amplitude modified signal and having second frequency different from the first frequency, means for modulating the gain of the detection means or amplifying the output therefrom with the signal, and means for deriving a generated signal from electromagnetic radiation and the detection means at a cross correlate frequency in order to detect the phase shift of luminescence and the variation in the modulation thereof.;COPYRIGHT: (C)1993,JPO
机译:目的:获得互相关频率范围的荧光测量设备和/或荧光测量设备,在其中可以以高灵敏度和高信噪比获取信号。组成:本发明的设备包括电磁辐射源10,用于以第一频率对电磁辐射进行幅度修改的装置12,用于将调制的电磁辐射引入样品20的装置,以及用于检测样品的发光的装置32。本发明的设备还包括用于向检测装置提供与调幅信号相干并具有不同于第一频率的第二频率的信号的装置26、28,用于调制检测装置的增益或用该信号放大其输出的装置。 ;以及用于从电磁辐射导出所产生的信号的装置和检测装置以互相关的频率检测发光的相移及其调制的变化。; COPYRIGHT:(C)1993,JPO

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