METHOD FOR PRODUCING PROTEIN DOMAIN AND METHOD FOR ANALYZING THREE-DIMENSIONAL STRUCTURE OF PROTEIN USING THE RESULTANT DOMAIN

摘要

PROBLEM TO BE SOLVED: To provide a method for quickly and easily selecting a protein domain highly soluble and suitable for analyzing the three-dimensional structure of protein. SOLUTION: This method for producing a soluble protein domain comprises the steps of (a) partially splitting a DNA encoding a protein to prepare at least two DNA fragments, (b) expressing proteins encoded by the above respective DNA fragments as fused proteins with functional proteins, (c) selecting a protein having the above function among the thus expressed two or more fused proteins, and (d) synthesizing the objective soluble protein domain included in the thus selected functional fused protein and encoded by the DNA fragment with a cell-free protein synthetic system. By using this method, the three- dimensional structure of a protein intended for many clones can be analyzed easily and quickly.