Biological activity of cystein - knot family rearrangement human growth factor production manner of the dimer and use of the dimer which is generated

摘要

(57) Abstract As for this invention the cell enclosure body the cystein - knot family rearrangement human growth factor which is used as the starting raw materials biological activity in regard to the production manner of the dimer, manner the restoration with especially, treat (ii) aforementioned solubilization ones with the ion exchange column thing and the (iii) regeneration which make the aforementioned cell enclosure body solubilize in the urea content water alkaline nutrient medium (A) as for (i) itself under existing of the known reducing agent and the elution with dimer conversion and aerobic re-oxidation and the (iv) inorganic salt gradient in the time of cleaning with drop urea gradient or (B) as for (i) itself the known reducing agent as for itselfThing and (ii) aforementioned solubilization ones which make the aforementioned cell enclosure body solubilize in the water nutrient medium under existing of the known solubilizer, if it is treatment possible, while gel percolating treating under denaturation condition, (iii) itself under existing of the known solubilizer and gel percolating treating aforementioned solubilization ones that itself it is possible to make the monomer join, thing and the (iv) gel which are made to regenerate under conditional existing of the known redox medicine to dimer converting the aforementioned growth factor in the effluent which is percolated with the means of the redox medicine, activity make the dimer generate biologicallyIn each case on the one hand, and (C) being based on to (A) or (B), to dimer the aforementioned growth factor which it converts that itself furthermore treating with known refinement step in regard to the aforementioned refinement of the recombinant protein, refining precisely, with it features.