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IDENTIFICATION OF NUCLEOTIDE SEQUENCE SPECIFIC TO MYCOBACTERIUM AND DEVELOPMENT OF DIFFERENTIAL DIAGNOSIS STRATEGY FOR MYCOBACTERIAL SPECIES
IDENTIFICATION OF NUCLEOTIDE SEQUENCE SPECIFIC TO MYCOBACTERIUM AND DEVELOPMENT OF DIFFERENTIAL DIAGNOSIS STRATEGY FOR MYCOBACTERIAL SPECIES
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机译:菌种特异的核苷酸序列的鉴定和菌种特异的诊断策略的发展
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摘要
PROBLEM TO BE SOLVED: To provide a method for detecting non-tuberculous Mycobacterium strains(NTM) in a sample.;SOLUTION: This method comprises the steps of (i) providing a non- tuberculous Mycobacterium species-specific upstream p34 gene region (us-p34) nucleotide probe, (ii) reacting the us-p34 nucleotide probe with the above sample under conditions that allow for the selective formation of nucleotide duplexes between the us-p34 nucleotide probe and the corresponding no-tuberculous Mycobacterium nucleic acid target present in the sample, and (iii) detecting any nucleotide duplexes containing the p34 nucleotide probe.;COPYRIGHT: (C)2002,JPO
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