ADSORBER USED FOR REFINING GLYCOSIDASE, PREPARATION METHOD THEREFOR, AND METHOD OF REFINING GLYCOSIDASE

摘要

PROBLEM TO BE SOLVED: To refine a glycosidase of a refining object contained in a crude enzyme solution, using reduced processes. SOLUTION: An adsorber containing an inhibitor containing at least one kind of a glucoamidine derivative exhibiting a selective (specific) inhibition effect strongly to the glycosidase is filled in a column, the crude enzyme solution containing the glycosidase of the refining object is made to flow through the column, the column is washed thereafter with a citric acid buffer solution containing sodium chloride, and then a solution containing a saccharide or a structural analogue serving as a substrate for the glycosidase of the refining object such as a citric acid buffer solution containing glucose is made to flow through the column, to refine the glycosidase of the refining object contained in the crude enzyme solution.