DETECTION OF MEC A GENE OF METHICILINRESISTANT STAPHYROCOCCUS AUREUS(MRSA)

摘要

PROBLEM TO BE SOLVED: To provide a combination of oligonucleotides that can specifically achieve the amplification, detection and identification of the RNA originating from the mec A gene of MRSA with high sensitivity.;SOLUTION: In the detection process utilizing the RNA amplification technique, characteristically the oligonucleotide of sequence number 1 is used as the first primer, any of oligonucleotides of the sequence number 2 to 4 is used as the second primer, or No.5 oligonucleotide is used as the first primer and either of No.6 or 1 oligonucleotide, as the second primer or No.8 oligonucleotide is used as the first primer and either of No.6 or 7 oligonucleotide, as the second primer.;COPYRIGHT: (C)2001,JPO