首页> 外国专利> DISPLAY METHOD FOR CORRESPONDENCE BETWEEN cDNA SEQUENCE AND GENOME SEQUENCE, RECORDING MEDIUM, SEQUENCER DEVICE AND PRIMER DESIGN METHOD

DISPLAY METHOD FOR CORRESPONDENCE BETWEEN cDNA SEQUENCE AND GENOME SEQUENCE, RECORDING MEDIUM, SEQUENCER DEVICE AND PRIMER DESIGN METHOD

机译:cDNA序列与基因组序列对应的显示方法,记录介质,序列装置及引物设计方法

摘要

PROBLEM TO BE SOLVED: To provide a display method for displaying a correspondence relation between a cDNA sequence that has an exon/intron structure and a genome sequence graphically in a plain manner. SOLUTION: From a similarity retrieval result regarding a cDNA and a genome, information on base position, similarity or the like of both ends of a pair (exon) of partial sequences that has a similarity is extracted. Among the pieces of the extracted information, information of a partial sequence pair that is judged to be unlikely to have significance in terms of similarity/sequence length or the like is eliminated. Moreover, the consistency in orientation/order between the exons is examined to select only an exon that covers the cDNA by a predetermined ratio or more and clearly shows relevance to the cDNA. Regarding the selected exon, its base position on the genome sequence is displayed on an axis 1 in a graph as a line segment, and its base position on the cDNA sequence is displayed on the other axis as a line segment, so that an intron/exon structure can be visually confirmed as line segments in juxtaposition.
机译:解决的问题:提供一种显示方法,以简单的方式以图形方式显示具有外显子/内含子结构的cDNA序列与基因组序列之间的对应关系。解决方案:从有关cDNA和基因组的相似性检索结果中,提取有关具有相似性的部分序列对(外显子)两端的碱基位置,相似性等信息。在所提取的信息中,消除在相似度/序列长度等方面被判断为不具有重要性的部分序列对的信息。此外,检查外显子之间的方向/顺序的一致性,以仅选择以预定比率或更大比例覆盖cDNA并且清楚地显示与cDNA相关的外显子。关于所选外显子,其在基因组序列上的碱基位置在图的轴1上显示为线段,在cDNA序列上的碱基位置在另一轴上显示为线段,因此内含子/外显子结构可以在视觉上确认为并列的线段。

著录项

  • 公开/公告号JP2002099546A

    专利类型

  • 公开/公告日2002-04-05

    原文格式PDF

  • 申请/专利权人 HITACHI LTD;

    申请/专利号JP20000289728

  • 发明设计人 KIMURA KOICHI;NISHIKAWA TETSUO;

    申请日2000-09-25

  • 分类号G06F17/30;C12M1/00;C12N15/09;

  • 国家 JP

  • 入库时间 2022-08-22 00:53:47

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