The invention is in the field of immunologic serological in vitro diagnostics. The invention is an ELISA-based diagnostic testing system and method that provides the capability to “look within” and measure an immune complexes specific antigen and antibody using typical ELISA microplates and procedures. One aspect of the invention is a method for detecting antigen and antibody in immune complexes. A second aspect of the invention is for a well design that may be used in the method of the invention. A third aspect of the invention is for a kit for detecting antigen, antibody, or both antigen and antibody in immune complexes.
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