Immunological detection of neosporosis using a recombinant antigen

摘要

An immunological assay method is disclosed which utilizes recombinant antigen, rNcp29, derived from an immunodominant surface antigen of Neospora caninum tachyzoites. Specifically, an ELISA method is disclosed. The method provides sensitive and specific detection of antibodies in sera of infected animals and does not exhibit cross-reaction with antisera against related parasites such as T. gondii. The ELISA method is used to screen animals for the presence of serum antibodies specific to recombinant Ncp29.