Micronucleus assay with genomic DNA hybridization probe and enzymatic color pigment detection

摘要

A method for detecting the presence of micronuclei in cells of an organism comprises the steps of;(a) isolating cells of the organism,;(b) exposing the cells to a hybridization probe, the hybridization probe comprising digested, labeled whole genomic DNA, the digested genomic DNA being labeled with a first binding member capable of specifically binding with a second binding member, whereby, as a result of exposing the cells to the hybridization probe, the hybridization probe binds hybridizes with DNA in the cells, including DNA contained in micronuclei, if present,;(c) exposing the cells to a compound comprising the second binding member coupled to an enzyme capable of reacting with a chromogenic substrate to convert the chromogenic substrate into a colored pigment, whereby, as a result of exposing the cells to the compound, the compound binds to the hybridization probe that is hybridized with the DNA in the cells,;(d) exposing the cells to the chromogenic substrate, whereby the chromogenic substrate is converted into a colored pigment in the presence of the enzyme, and;(e) examining the cells and scoring the cells for the presence or absence of micronuclei.