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Phytase having a low Michaelis constant for phytic acid from monascus

机译:对来自红曲霉的植酸具有低米氏常数的植酸酶

摘要

Provided is an isolated phytase derived from a microorganism. The microorganism is preferably from the genus Monascus. The phytase has a Michaelis constant of 10 to 110 &mgr;M when phytic acid is used as a substrate. A process for producing the phytase is also provided as is an animal feed comprising the phytase. The phytase is preferably isolated from Monascus anka IFO 30873. The phytase has a Km of 107 &mgr;m when phytic acid is used as a substrate, the optimum pH is 2.5, the optimum temperature is 45° C., the molecular weight of the phytase is 140 kDa when measured by gel filtration, and the isolectric point is 5.2 using chromatofocusing.
机译:提供了衍生自微生物的分离的植酸酶。微生物优选地来自红曲菌属。当将植酸用作底物时,该植酸酶的米氏常数为10至110μM。还提供了产生肌醇六磷酸酶的方法以及包含肌醇六磷酸酶的动物饲料。植酸酶优选地从
红曲霉IFO 30873中分离出来。当以植酸为底物时,植酸酶的Km为107μm,最适pH为2.5,最适温度为45℃。 C.,通过凝胶过滤测量时,植酸酶的分子量为140 kDa,使用色谱聚焦法测得的等电点为5.2。

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