Promoter for amp;prime;-pyrroline-5-carboxylate synthetase gene in arabidopsis thaliana

摘要

A genomic DNA library of A. thaliana, Columbia ecotype is screened by a plaque hybridization using the full-length P5CS1 cDNA as a probe. Positive clons are purified. These clones are subcdoned and sequence thereof is determined by cycle sequencing methods. Then deletion series of DNA is combined to GUS gene and introduced to A. thaliana. Using the transgenic A. thaliana plants, an analysis of the activity of GUS gene is determined and the activity region of the P5CS1 promoter is checked. It becomes clear that the P5CS1 promoter of A. thaliana controls expression of gene under water stress.