A process for the cloning of mRNAs and display of differentially expressed transcripts (DODET)

摘要

The present invention relates to a two-step reverse transcription of DNA from RNA, wherein two types of reverse transcriptase enzymes are employed. The one type of reverse transcriptase enzyme having a temperature optimum at below 55 DEG C and the other type of reverse transcriptase enzyme having a temperature optimum above 55 DEG C, thereby obtaining cDNAs which are representatives of the entire gene, including the translated regions and at the same time performed effectively. IMAGE IMAGE