An improved process for the preparation of technical grade azadirachtin

摘要

An improved process for preparation of technical grade azadirachtin, characterized in four step process of preparation 0.21-0.42% oil-free powder of neem containing 27-40% azadirachtin from non aqueous extract of seed kernel of Azadiracta indica (neem), the said process comprising the step of: (i) obtaining filtrate of said Azadiracta seed kernel powder by mechanical stirring in «powdef by mechanical stirring in polar solvent and concentrating in rotary evaporator, (ii) centrifuging the obtained filtrate, diluted with non polar solvent, under low temperature at range 4-5°C, filtering and concentrating by the method as defined above, (iii) adding molecular sieve having mesh size 4-5 A0 to the filtrate obtained at step (ii) and diluting with less polar solvent, cooling the filtrate at a temperature 0-10° C for period 8-12 hours (iv) precipitating with non-polar solvent, filtering and drying by conventional method such as herein described to get the desired technical grade azadirachtin powder.