Method for producing a heterologous protein secreted from the cells of ovary hu00c1mster Chinese cultured on microcarriers.

摘要

The present invention relates to a method for producing a heterologous secreted protein from transformed CHO cells grown on microcarriers in a serum-free cell culture medium. This method avoids a production lag phase when switching from a serum-containing to a serum-free environment, a problem observed in the mammalian adherent cell culture production of secreted proteins.