Vector containing a nucleic acid insertion expressing a hybrid polypeptide with a protease inhibitor domain and a receptor binding domain

摘要

A recombinant nucleic acid molecule is provided comprising a vector useful for transfection or transduction of mammalian cells, wherein the vector contains a nucleic acid insertion encoding an expressible hybrid polypeptide or protein which comprises a domain with a receptor binding function and a domain with enzymatic activity, in particular protease inhibitor activity. The vector may be a viral (e.g. adenovirus or retrovirus) or non-viral vector, and the nucleic acid insertion encoding the expressible hybrid polypeptide or protein may be under the control of a cell- or tissue-specific promoter. The vector may be used to express the hybrid polypeptide in a patient in a method to prevent local proteolytic activity, extracellular matrix degradation, cell migration, cell invasion or tissue remodelling.