METHOD FOR PRODUCING DIAGNOSTIC REAGENTS, TEST METHODS AND MEDICINES BASED ON THE CLINICAL MANIFESTATIONS OF A DISEASE

摘要

Antibodies are recovered and isolated from the sera of a number "n" of patients each of which have or have had the same given disease so that each of the "n" patients has, within a large number of antibodies, some antibodies uniquely associated with the same disease of interest. The antibodies of a first patient are bound to a support surface. To carry out initial screening libraries of molecules, such as peptides displayed on the surface of bacteriophage, are brought into contact with the bound antibodies of the first patient under conditions where binding will occur. Unbound molecules (peptides) are washed away and bound molecules (peptides) on the bacteriophage are identified. Secondary screening is then carried out by extracting and labeling the antibodies of a second patient and using the labeled antibodies to probe the molecules (peptides) isolated in the initial screening.