Procedures for determining the endogenous thrombin potential (ETP) and thrombin substrates for use in such procedures.

摘要

A method for determining ETP (Endogenous thrombin potential) of a sample, preferably in a continuous testing, said SHOWS OR EQUAL TOTAL anticoagulant activity equivalent to at least 0.07 U ISH / ml, in which a thrombin substrate or a salt thereof which is soluble in the sample, applied in a manner known per se, TO DETERMINE THE ETP of a sample being selected SAID thrombin substrate between the group of substrates of formula P INO, REMAIN This group nonaromatic AND POLAR, VAL IS A RADICAL VALINE linked through a peptide bond A XAA, Xaa is an amino acid residue comprising a guanidino group or a ureido TERMINAL, separated by at least 2 carbon atoms SKELETON peptide, said AS KINGDOM amino acid residue, S is a group signal such as a chromophore, which can be enzymatically hydrolysed. Can also be used OTHER substrates such as substrates include the structure ZAA pipecolyl YAA resents {SUB, D} Tyrosine, PRO REPRESENT Proline, YAA IS A SEPARATE amino acid residue arginine is a marker YS SIGNAL. ALSO APPLY THE COMPLETION OF ETP SUSTRATOS BOC as such may be improved by the addition of hydroxylamine to the sample, TO PREVENT SAMPLE Defibrination.