Fluorescent labeling complexes with large stokes shifts formed by coupling cyanine and other fluorochromes capable of resonance energy transfer.

摘要

The present invention provides COMPLEJOS FLUORESCENT LABELING low molecular weight DEVIATIONS WITH LARGE wavelength between the absorption of DYE IN THE COMPLEX AND ISSUANCE OF OTHER DYE IN THE COMPLEX. These complex CAN BE USED FOR EXAMPLE FOR CELLULAR ANALYSIS OF MULTIPLE FLUORESCENCE PARAMETERS USING A EXCITATION WAVELENGTH SIMPLE. THE COMPLEX low molecular weight allows materials LABELED WITH COMPLEX FROM ENTERING THE CELLULAR STRUCTURES FOR USE AS PROBES. LABELING COMPLEXES synthesized by joining them covalently bound through linkers for complexing donor- acceptor. TRANSFER FROM A RESONANT POWER EXCITED GIVING FLUORESCENT PROVIDES AN Acceptor CHANGES TO WAVELENGTH 300nm. LABELING FLUORESCENT COMPLEXES CONTAINING PREFERABLY FOR GROUPS REACTIVE FUNCTIONAL GROUPS ON LABELING OF TARGET COMPOUNDS SUCH AS ACIDOSPOLINUCLEICOS OXI AND deoxy, antibodies, enzymes, lipids, carbohydrates, proteins and other materials. The complexes may contain functional groups that allow covalent reaction with materials containing reactive groups.