Cetp taqib polymorphism as risk factor for development of coronary heart disease

摘要

Disclosed is method for assessing risk for the development of cardiovascular disease in an individual. The method includes isolating nucleic acid from the individual, analyzing the nucleic acid for the presence of the TaqIB polymorphism of the cholesteryl ester transfer protein gene, determining from the analysis whether the individual is homozygous for the TaqIB polymorphism; is heterozygous for the TaqIB polymorphism; or does not possess the TaqIB polymorphism. Risk for the development of cardiovascular disease is assessed in the individual on the basis of these determinations. Additional determinations of one or more known factors of cardiovascular disease risk may also be assessed. Methods for analysis of genomic DNA for the presence of the TaqIB polymorphism are provided. Also disclosed is a kit for assessing risk for the development of cardiovascular disease in an individual. The kit contains useful reagents, such as oligonucleotide primers for the amplification of a suitable section of the first intron of the cholesteryl ester transfer protein gene encompassing the TaqI restriction site of the B1 allele of the CETP gene. Optionally, the kit also contains indicators for additional known factors of cardiovascular disease risk.