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A novel assay for determination of androgenic or anti-androgenic activity of a serum sample or a test compound
A novel assay for determination of androgenic or anti-androgenic activity of a serum sample or a test compound
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机译:一种测定血清样品或测试化合物的雄激素或抗雄激素活性的新方法
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摘要
The present invention is directed to methods for determining the androgenic or anti-androgenic activity of a sample and to a cell useful in these methods. To determine androgenic activity, the sample is contacted and incubated with a cell comprisingi) a luciferase reporter plasmid,ii) a fusion protein comprising a ligand-binding domain of the androgen receptor and a Gal4 DNA-binding domain, said fusion protein being able to bind to binding sites in said luciferase reporter plasmid,iii) a fusion protein comprising an N-terminal region of the androgen receptor and a transcriptional activation domain, andiv) an androgen receptor-interacting protein 3.The cell is then lysed and the luciferase activity of the lysate is measured. The measured luciferase activity is compared to that obtained by repeating the above method except that a control is added instead of the sample, to give the relative luciferase activity of the sample. The relative luciferase activity is used to detect or quantify an active androgen in the sample.
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