A fibrinogen clotting inhibitor derived from snake venom and process for preparing the same

摘要

The present invention relates to Salmorin protein derived from snake venom of Agkistrodon halys brevicaudus with fibrinogen clotting inhibitory activity, cDNA thereof and process for preparing the same. The protein of the present invention is prepared by the process comprising the step of collecting snake venom of Agkistrodon halys brevicaudus and purifying Salmorin protein from the venom through anion exchange chromatography, gel filtration and anion exchange chromatography again. The Salmorin protein is 27kDa single band-protein under non-reducing SDS-PAGE but can be separated into 15kDa A chain and 14kDa chain under reducing SDS-PAGE and Salmorin A chain and B chain have seven Cys residues respectively. The Salmorin protein dose-dependently represses fibrinogen clotting by potently repressing the activation of prothrombin into thrombin through two molecular complex with prothrombin and delays the blood coagulation by competitively functioning with hirudin. Therefore the Salmorin protein can be widely applied to the effective materials of antithrombotic agents.