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AUTOMATED PLANT EXTRACTION METHOD AND ARRAYS FOR HIGH THROUGHPUT SCREENING
AUTOMATED PLANT EXTRACTION METHOD AND ARRAYS FOR HIGH THROUGHPUT SCREENING
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机译:自动化植物提取方法和阵列进行高通量筛选
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摘要
An automated and validated method for preparing plant extracts for use in the high throughput screening of biological assays uses a robotic sample processor for the aspiration and dispensing of solvent extracts and a fully automated solid phase extraction apparatus to produce column fractionated plant extracts. The method comprises the steps of: (a) thoroughly extracting a biological source material with at least one solvent to produce at least one solvent extract containing the potentially bio-active biochemicals of the source material; (b) removing from the solvent extracts interferences, comprising non-selectively bio-active compounds that interfere with biological assays; (c) subjecting the solvent extracts to chromatography by an automated system for handling samples; (d) detecting the potentially selectively bio-active biochemicals of the source material in the eluent; and (e) collecting the biochemicals in isolated fractions having standardized concentrations under control by the automated system with associated data.
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