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POLYMER ARRAYS AND METHODS OF USING LABELED PROBE MOLECULES TO IDENTIFY AND QUANTIFY TARGET MOLECULE EXPRESSION
POLYMER ARRAYS AND METHODS OF USING LABELED PROBE MOLECULES TO IDENTIFY AND QUANTIFY TARGET MOLECULE EXPRESSION
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机译:聚合物阵列和使用标记探针分子识别和量化目标分子表达的方法
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摘要
An assay system using labeled probe molecules to identify and quantify target molecules in a sample is disclosed. Where labeled probe molecules are present on a substrate such as a microarray, the identification of multiple different target molecules may be examined simultaneously. Alternatively, a known number of like probe molecules may be present on a substrate and a single target molecule may be quantified in a sample. Preferred labeled probe molecules are comprised fluorescent single stranded nucleotide analogs whose fluorescence is quenched by pairing with a homologous nucleotide target sequence.
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