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A METHOD OF MAPPING RESTRICTION ENDONUCLEASE CLEAVAGE SITES
A METHOD OF MAPPING RESTRICTION ENDONUCLEASE CLEAVAGE SITES
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机译:映射限制性内切酶裂解位点的方法
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摘要
The invention provides a two-step sorting procedure where it is possible to scan the overhanging single-stranded ends of nucleic acid fragments quickly and efficiently using solid supports, such as microarrays. Use is made of two different sets of degenerate overhang-adaptors in this regard. The invention also provides new methods and strategies inter alia for collecting information about sequences and cleavage sites that are between the cleavage sites that have generated an overhang pair. An effective method of producing the restriction map, making it easier to create multiple maps, is also described.
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