IRON REGULATION OF THE PpSEC10 TRANSCRIPTIONAL REGULATORY REGION OF iPICHIA PASTORIS/i AND METHODS OF USE

摘要

Methods and compositions for the expression of nucleotide sequences are provided. Compositions comprise the nucleic acid sequences of the transcriptional regulatory region of the PpSEC10 gene of iPichia pastoris/i. Methods are provided to chemically regulate the PpSEC20 transcriptiona control region by modulating the iron concentration in he culturing medium. The methods find use in regulating expression of nucleotide sequences. Furthermore, the methods of the invention can be used to regulate polypeptide expression, more particularly in regulating heterologous polypeptide expression, particularly using a yeast host cell as the expression system.