MEIOTIC SPINDLE IMAGING IN OOCYTES AND USES THEREFOR IN IN VITRO FERTILIZATION

摘要

The development potential of a mammalian producing for successful implantation is evaluated by using polarized light optics (fig.1), and calculating a retardance image with a sensitivity of 3nm o r less. The presence, location and morphological characteristics of a meiotic spindle in the oocyte can then be determined based on the spindle structure in the retardance image (fig.2), and the developmental potential of the oocyte can be evaluated.