AN ARTIFICIAL GENE AND VECTORS FOR EXPRESSING HIGH-YIELD RECOMBINANT OVINE INTERFERON-TAU IN PICHIA PASTORIS

摘要

Methods and expression vectors encoding for the ovine interferon-tau (OvIFN-tau) protein are provided for high yield production in any industrial yeast, such as Pichia pastoris. Compared to previous studies, significantly higher levels of correctly-folded and biologically active OvIFN-tau polypeptides are produced by using two different Pichia pastoris expression vectors comprising an optimized IFN-tau nucleotide sequence fused to the α-MF pre-pro signal sequence under the control of the Pichia pastoris AOX1 promoter.