Immunological ELISA test for inter-alpha-trypsin inhibitor

摘要

Sandwich immunoassay for inter- alpha -trypsin inhibitor (ITI) uses: (a) an immunoglobulin which is specific for 1 of the 2 heavy chains of ITI and is immobilised on a support, and(b) an immunoglobulin which is specific for the other heavy chain of ITI and is labelled with biotin. Also claimed are:(1) immunoglobulins specific for heavy chain H1 of ITI, obtd. by immunising an animal with a high-purity H1 prepn., recovering the crude antiserum and purifying the anti-H1 immunoglobulins by eliminating contaminating immunoglobulins, esp. anti-H2 and anti-bikunin immunoglobulins, by adsorption on prepns. of H2 and bikunin immobilised on CNBr-activated 'Sepharose' (RTM), and(2) immunoglobulins specific for heavy chain H2 of ITI, obtd. by immunising an animal with a high-purity H2 prepn., recovering the crude antiserum and purifying the anti-H2 immunoglobulins by eliminating contaminating immunoglobulins, esp. anti-H1 immunoglobulins, by adsorption on a prepn. of H1 immobilised on CNBr-activated 'Sepharose' (RTM).