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METHOD FOR SCREENING OF DNA LIBRARIES AND GENERATION OF RECOMBINANT DNA CONSTRUCTS
METHOD FOR SCREENING OF DNA LIBRARIES AND GENERATION OF RECOMBINANT DNA CONSTRUCTS
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机译:DNA库的筛选和重组DNA结构的生成方法
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摘要
A method of DNA library screening includes homologous recombination in E. coli utilizing lambda phage recombination functions. Inserting a positive selection marker such as antibiotic resistance into the target sequence by homologous recombination facilitates isolation of target sequences and requires only about 58-100 base pairs of total homology, thus allowing the use of synthetic fragments of DNA for taargeting. DNA vector is designed for genomic library construction that features a novel genetic selection for inserts, automatic subcloning of isolated genomic clones and the presence of a negative selection marker adjacent to the genomic inserts to facilitate later gene targeting.
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