Isolation and purification of germinal stem cell using magnetic bead functioning as antibody and method for establishing stem cell line

摘要

Establishing a method for isolating and purifying undifferentiated germinal stem cell from cell populations as the origin of ES cell or EG cell and a method for generating a stem cell line by growing the cell in vitro.;A method for isolating and purifying germinal stem cell from an embryo or a fetus of a mammalian animal including humans, by utilizing an immunomagnetic bead and an antibody recognizing undifferentiated germinal stem cell, and a method for generating a stem cell line with infinite growth potency and pluripotency, comprising culturing the germinal stem cell in vitro, thereby establishing a cell line thereof.