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DETECTION OF THE END-POSITION SIALIC ACID GROUPS OF THE HUMAN TRANSFERRIN MOLECULE
DETECTION OF THE END-POSITION SIALIC ACID GROUPS OF THE HUMAN TRANSFERRIN MOLECULE
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机译:人类运铁蛋白分子末端唾液酸基团的检测
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摘要
The subject matter of this invention is a method of determining terminal sialic acid residues in human transferrin according to a sandwich principle, which is characterized in that the sample fluid containing the human transferrin is incubated with a first receptor which binds specifically to human transferrin, the thus-formed complex is separated from the sample fluid and incubated with a second receptor which binds specifically to terminal sialic acid residues in human transferrin, the second receptor being bound or able to bind to a marker, and the complex made up of the first receptor, human transferrin and the second receptor is determined with the marker. According to one embodiment, the method of the invention allows the determination of sialic-acid-deficient human transferrin in body fluids.
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