首页> 外国专利> METHODS FOR MAKING POLYNUCLEOTIDE LIBRARIES, POLYNUCLEOTIDE ARRAYS, AND CELL LIBRRARIES FOR HIGH-THROUGHPUT GENOMICS ANALYSIS

METHODS FOR MAKING POLYNUCLEOTIDE LIBRARIES, POLYNUCLEOTIDE ARRAYS, AND CELL LIBRRARIES FOR HIGH-THROUGHPUT GENOMICS ANALYSIS

机译:进行高通量基因组学分析的多核苷酸文库,多核苷酸文库和细胞文库的制备方法

摘要

A method for high-throughput, genomics analysis, to identify the therapeutic or diagnostic utility of genes, entails the use of a construct to disrupt a gene or alleles of a gene in cells of interest. Arrays of such cells can be used to monitor such disrupted cells phenotypically in the context, for example, of testing drug candidates. Polynucleotides that comprise part of the disrupted genes can be recovered from such 'knockout' cells, by virtue of an origin of replication or a host cell selection marker sequence that is part of the construct. The recovered polynucleotides can be used to identify the disrupted genes or to make homologous recombination vectors, whch in turn can be employed to make multi-allele knockout cells.
机译:一种用于鉴定基因的治疗或诊断用途的高通量基因组学分析的方法,需要使用构建体来破坏目的细胞中的一个基因或该基因的等位基因。这种细胞的阵列可以用于例如在测试候选药物的情况下在表型上监测这种破坏的细胞。通过复制起点或作为构建体一部分的宿主细胞选择标记序列,可以从此类“敲除”细胞中回收包含部分破坏基因的多核苷酸。回收的多核苷酸可用于鉴定被破坏的基因或制备同源重组载体,而后者又可用于制备多等位基因敲除细胞。

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