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METHODS FOR MAKING POLYNUCLEOTIDE LIBRARIES, POLYNUCLEOTIDE ARRAYS, AND CELL LIBRRARIES FOR HIGH-THROUGHPUT GENOMICS ANALYSIS
METHODS FOR MAKING POLYNUCLEOTIDE LIBRARIES, POLYNUCLEOTIDE ARRAYS, AND CELL LIBRRARIES FOR HIGH-THROUGHPUT GENOMICS ANALYSIS
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机译:进行高通量基因组学分析的多核苷酸文库,多核苷酸文库和细胞文库的制备方法
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摘要
A method for high-throughput, genomics analysis, to identify the therapeutic or diagnostic utility of genes, entails the use of a construct to disrupt a gene or alleles of a gene in cells of interest. Arrays of such cells can be used to monitor such disrupted cells phenotypically in the context, for example, of testing drug candidates. Polynucleotides that comprise part of the disrupted genes can be recovered from such 'knockout' cells, by virtue of an origin of replication or a host cell selection marker sequence that is part of the construct. The recovered polynucleotides can be used to identify the disrupted genes or to make homologous recombination vectors, whch in turn can be employed to make multi-allele knockout cells.
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