METHOD FOR DETECTION AND QUANTIFICATION OF THE FUNGUS ASPERGILLUS FUMIGATUS USING QUANTITATIVE PCR

摘要

A method for detecting the fungus Aspergillus fumigatus includes isolating DNA from a sample suspected of containing the fungus Aspergillus fumigatus. The method further includes subjecting the DNA to polymerase chain reaction amplification utilizing at least one primer. The at least one primer includes one of a 5'CGCGTCCGGTCCTCG3' and 5'TTAGAAAAATAAAGTTGGGTGTCGG3' base sequence. The method also includes detecting the fungus Aspergillus fumigatus by visualizing the product of the polymerase chain reaction.