DNA BINDING PROTEIN AND SEQUENCE AS INSULATORS HAVING SPECIFIC ENHANCER BLOCKING ACTIVITY FOR REGULATION OF GENE EXPRESSION

摘要

(57) insulator elements that it is necessary and sufficient for enhancer blocking activity in the [summary] human cells has been shown is described. For the control of gene expression, an insulator element of the invention, a 42 base pair DNA molecule having enhancer blocking activity of certain. It also insulator element is a DNA-binding protein that is highly conserved in vertebrates, is the center of the CTCF binding sites have also been shown. Moreover, insulator elements, including a CTCF binding site has been identified in the region of Igf locus is methylated allele father on exclusively. Methylation of the insulator sequences results in the deletion of the enhancer blocking activity, and abolished the ability to bind CTCF insulator.