Methods of detecting poly(ADP-ribose) polymerase enzymatic activity

摘要

The invention provides methods for assaying poly(ADP-ribose) polymerase (PARP) activity and methods for the identification of modulators of PARP activity. These methods comprise contacting immobilized PARP with NAD under conditions that allow PARP auto-ribosylation, contacting the auto-ribosylated PARP with a detectable marker to form a complex and then indirectly or directly measuring the amount of detectable marker bound to the auto-ribosylated PARP, this amount being indicative of PARP activity.