Method for screening anti-cervical cancer agents and anti-apoptotic modulating agents

摘要

The invention papil person causing cervical cancer by the present invention using the transformant Escherichia coli BL21 / pET28a-E6 (E.coli BL21 / pET28a-E6) KCTC 18015P relates to cervical cancer and apoptosis regulators search method Roman E6 protein of the virus type 16 (His-16 E6) a mass expression and a cost-soluble His-16 E6 protein purified using nickel beads after, E6- binding proteins involved in the ubiquitin (ubiquitin) reaction (E6- associated protein; a bond with the soluble fraction of the E6AP) by identifying a Western blotting method and Ella and (ELISA) method in vitro, and the excellent effect of establishing the E6-E6AP in vitro binding capacity search system E6-E6AP coupled to the search system the screening of synthetic peptide capable of competitively inhibiting and variations, cell run, which can be screened for anticancer drug design based on the combined navigation and E6-p53 binding structure of apoptosis regulators I have worked.