Diagnosis method of Helicobacter pylori infection and rapid urease reaction kit for it

摘要

The present invention provides a method for obtaining gastrointestinal tissue from (a) humans or animals;;(b) contacting the sample with an acidic buffer of pH 2.0 to 5.0, particularly a test solution comprising 0.1 to 2 mL of HCl-KCl buffer and an effective amount of indicator that changes color at pH 3.0 to 5.0; In addition;(c) measuring a color change of the test solution;;H. In human or animal stomach tissue due to the color change of the test solution. A detection method for confirming the presence of pylori. The test solution may further comprise 50 to 500 mg / dL of urea. The present invention also provides an effective amount of color changing in (a) from 50 to 500 mg / dL of urea, (b) from 0.1 to 2 ml of acidic buffer at pH 2.0 to 5.0, in particular from 0.1 to 2 ml of HCl-KCl buffer and (c) at pH 3.0 to 5.0 H on the specimen by color change by the indicator, including the indicator. H present in the gastrointestinal tract of humans or animals to confirm the presence of pylori. A rapid urease test kit for diagnosing pylori.